Application

Flow Cytometry (5 µg/ml)r>Frozen Sections (see comments)r>Immunoprecipitation (see comments)r>T cell Co-stimulation (see application references)

General description

Protein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.

Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Recognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.

Immunogen

human thymocytes/Sezary T cells

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 10⁵ cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Thibault, G. and Bardos, P. 1995. J. Immunol.154, 3814.Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol.11, 329.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)